Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP-tag antibody is suitable for detecting fusion proteins that contain a MBP-tag.
Full-length MBP protein
1mg/ml in PBS, pH 7.4, containing 0.1% proclin and 50% glycerol
WB: 1:1000~1:10000Optimal dilutions/concentrations should be determined by the end user.
Fig: Western blot analysis of MBP-tag fusionprotein with anti MBP-tag mouse mAb (6D3)diluted at 1: 2000(left) and 1: 5000(right).